FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001283709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001283709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18673806
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC883927847.335170987639046RNA PCR Primer, Index 11 (100% over 51bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG10530755.639316377175601RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCCA5633183.0166212501083067RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG3394341.8177012227716192RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2490891.333895189871845RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1609450.8618757204610565RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1477340.7911295640535196RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1276330.6834868049930475RNA PCR Primer, Index 1 (100% over 29bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1241190.664669002130578RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC1216750.6515811506234991RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1062900.5691930182845425RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG816040.43699714991148564RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC812580.43514428713675185RNA PCR Primer, Index 1 (100% over 31bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA791240.4237165149943187RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG787770.42185829712486034RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC756130.4049147774160233RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC722200.38674494101523815RNA PCR Primer, Index 1 (100% over 31bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA695060.37221121393249984RNA PCR Primer, Index 1 (100% over 23bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG680630.36448381224480964RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG576620.3087854720135788RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC509050.27260109695902374RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG490800.26282804908651186RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT437370.23421577797263182RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG409670.21938216558531237RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT386300.2068673092137725RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG380070.20353108520030677RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA378190.20252432739206994RNA PCR Primer, Index 11 (100% over 38bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG375650.20116413333200528RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC374370.20047868120724827RNA PCR Primer, Index 1 (100% over 27bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC369120.19766725647679964RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACACTCGTATGCCG365550.19575548766009457RNA PCR Primer, Index 11 (97% over 40bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT357390.1913857303647687RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTTACATCTCGTATGCC319730.17121844363168387RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTAGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC310660.16636137271641357RNA PCR Primer, Index 11 (98% over 51bp)
CATAAAGTAGACAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT307790.16482446053043498RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA303020.16227008034677023RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG275200.14737220682275481RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA257670.13798472577041873RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG256090.1371386208039218RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT250910.13436468173654584RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAACTATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG247840.132720667656074RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAATCCAGTCACGGCTACATCTCGTATGCCG245960.13171390984783712TruSeq Adapter, Index 11 (96% over 30bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACGGCTACATCTCGTATGCCG242990.1301234467146119Illumina PCR Primer Index 11 (96% over 32bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG241630.12939515383205757RNA PCR Primer, Index 11 (98% over 51bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC239670.12834555526602343RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG235580.12615532152363584RNA PCR Primer, Index 1 (100% over 29bp)
TGTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCCA230170.12325821527759259RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTCAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG225170.12058066791526055RNA PCR Primer, Index 1 (100% over 29bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA222130.11895271911896267RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC222020.11889381307699139RNA PCR Primer, Index 1 (100% over 31bp)
GTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCCAG217170.1162965921355293RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTGCATCTCGTATGCC215520.11541300150595973RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACGGCTACATCTCGTATGCCG215360.11532731999036512RNA PCR Primer, Index 11 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACGGCTACATCTCGTATGCCG212840.11397783611974976TruSeq Adapter, Index 11 (96% over 33bp)
AAGCTGCCAGTTGCAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG211990.11352265306815333RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT211250.11312637605852818RNA PCR Primer, Index 1 (100% over 30bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGGA208090.11143416612553435RNA PCR Primer, Index 1 (100% over 22bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC201780.10805510135427134RNA PCR Primer, Index 1 (100% over 24bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA190840.10219662772548886RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph