FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001283716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001283716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25727018
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC697185027.099331916353464RNA PCR Primer, Index 2 (100% over 51bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG19944927.752519160984766RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCCA11134624.32798702127079RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG10070733.914456778473121RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT5870522.2818501545729086RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG3126751.2153565562864688RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC2776081.0790523798755067RNA PCR Primer, Index 1 (100% over 27bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2755091.0708936418515353RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2724691.059077270439971RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC2550460.9913546917874432RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTGTATCTCGTATGCC2534520.9851588707249321RNA PCR Primer, Index 2 (98% over 51bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG2451130.9527454755930127RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2272680.8833825980142743RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA2199500.8549377934123574RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2100910.816616212574656RNA PCR Primer, Index 1 (100% over 31bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1754580.6819989786612657RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1735050.6744077374221917RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1713400.6659924597557323RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1701570.6613941810123506RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1334040.5185365828251063RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA1272070.4944490651812037RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1011100.39301095836291633RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC934880.363384516619843RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG906710.3524349382427454RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG864950.336202975408965RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG851750.33107218255920684RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG773420.30062559135302813RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT749130.2911841551166171RNA PCR Primer, Index 1 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA736560.28629824101650647RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTTGTATCTCGTATGCC735530.2858978836956541RNA PCR Primer, Index 2 (98% over 51bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG722640.28088758673857966RNA PCR Primer, Index 1 (100% over 29bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA719000.27947273174061604RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG702260.2729659535356954RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG632120.2457027860749349RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT628430.2442684962555707RNA PCR Primer, Index 1 (100% over 30bp)
TCTCACACAGAAATCGCACCCGTGGAATTCTCGGGTGCCAAGGAACTCCAG605020.2351691128758102RNA PCR Primer, Index 1 (100% over 29bp)
CATAAAGTAGACAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT604200.23485038180484033RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC593440.23066800823943143RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG583350.2267460612807905RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA577720.22455770039108303RNA PCR Primer, Index 1 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC568760.22107498039609566RNA PCR Primer, Index 1 (100% over 24bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT536700.20861337291403145RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT528960.2056048625612187RNA PCR Primer, Index 1 (100% over 30bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC473030.18386507134250848RNA PCR Primer, Index 1 (100% over 31bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCG471290.18318873955776765RNA PCR Primer, Index 2 (98% over 51bp)
TCTCCCAACCCTTGTACCAGTATGGAATTCTCGGGTGCCAAGGAACTCCAG462430.17974488920558146RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG458200.17810070331509079RNA PCR Primer, Index 2 (100% over 38bp)
TAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT424810.16512212958377065RNA PCR Primer, Index 1 (100% over 30bp)
TACAGTACTGTGATAACTGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG424080.1648383811913219RNA PCR Primer, Index 1 (100% over 29bp)
GTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCCAG419790.1631708735151505RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT418250.16257228101601204RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG402500.1564503122748233RNA PCR Primer, Index 1 (100% over 29bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG395630.153779967814381RNA PCR Primer, Index 1 (100% over 29bp)
TGTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCCA393880.15309974906536RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT331090.12869350035048757RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG327110.1271464885669999RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC322190.12523410214118091RNA PCR Primer, Index 2 (100% over 34bp)
CCCATAAAGTAGACAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC320140.12443727446375635RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA310380.12064359732635939RNA PCR Primer, Index 1 (100% over 28bp)
ATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTAT302340.11751847804514304RNA PCR Primer, Index 2 (100% over 48bp)
TGTAAACATCCTTGACTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA290930.1130834518015263RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGCGTTGGAATTCTCGGGTGCCAAGGAACTCCA283880.11034314198404183RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT281050.10924313109276793RNA PCR Primer, Index 1 (100% over 30bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC279600.10867952127215055RNA PCR Primer, Index 1 (100% over 33bp)
TCTCCCAACCCTTGTACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA278720.1083374684155RNA PCR Primer, Index 1 (100% over 32bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC273030.1061257857401118RNA PCR Primer, Index 2 (100% over 50bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC272940.10609080306159073RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA272360.10586535913334379RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT271370.10548054966961191RNA PCR Primer, Index 1 (100% over 30bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT267910.10413566002869046RNA PCR Primer, Index 1 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG266460.10357205020807309RNA PCR Primer, Index 1 (100% over 29bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC265340.10313671020869966RNA PCR Primer, Index 2 (98% over 51bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCCA260270.1011660193186789RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTAGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC258730.10056742681954045RNA PCR Primer, Index 2 (98% over 51bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC258000.10028367842709171RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph