FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001283720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001283720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18579750
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG213690611.501263472328745RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC9329695.021429244204039RNA PCR Primer, Index 1 (100% over 27bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT6507603.502522908004683RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC4457072.398885883825132RNA PCR Primer, Index 13 (100% over 51bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG4297542.313023587507905RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG4280742.303981485219123RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA4261332.2935346277533335RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG3519281.8941481989800724Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG3121191.679888050162139RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2947651.5864852864005168RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC2895111.5582071879330992RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2189301.1783258655256397RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG2119291.1406450571186373RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG1922691.034830931524913RNA PCR Primer, Index 2 (97% over 35bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1919311.0330117466596698RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1894601.0197123212099195RNA PCR Primer, Index 1 (100% over 30bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1843380.9921446736366204RNA PCR Primer, Index 1 (100% over 31bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA1779650.9578438891804248RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG1373720.7393640926277264RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1341080.7217965796095211RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1330770.7162475275501554RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC1236680.6656063725292322RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCCA1227200.6605040433805622RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1212250.6524576487842947RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA1202530.647226146745785RNA PCR Primer, Index 1 (100% over 28bp)
TACAGTACTGTGATAACTGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG1103650.5940069161318101RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA1087080.5850886045291245RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1048560.5643563557098454RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1046330.5631561242750844RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1031540.5551958449387101RNA PCR Primer, Index 1 (100% over 29bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA1010490.5438663060590159RNA PCR Primer, Index 1 (100% over 22bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC925340.49803684118462305RNA PCR Primer, Index 13 (100% over 37bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT879190.47319797090918875RNA PCR Primer, Index 1 (100% over 30bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC865250.4656951788909969No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT833720.44872509048830045RNA PCR Primer, Index 1 (100% over 30bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT833060.4483698650412411RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT817470.439979009405401RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG709850.3820557327197621RNA PCR Primer, Index 1 (100% over 29bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA675560.36360015608390855RNA PCR Primer, Index 1 (100% over 34bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA646160.3477764770785398RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG635220.34188834618334474RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGGTGGAATTCTCGGGTGCCAAGGAACTCC629530.33882587225339417RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC625630.3367268127934983RNA PCR Primer, Index 1 (100% over 33bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC576720.3104024542849069RNA PCR Primer, Index 1 (100% over 24bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG575430.30970815000201835RNA PCR Primer, Index 2 (97% over 35bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC567350.3053593293774136RNA PCR Primer, Index 1 (100% over 27bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGGA561430.3021730647613665RNA PCR Primer, Index 1 (100% over 22bp)
TGAGAACTGAATTCCATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG555560.2990137111640361RNA PCR Primer, Index 1 (100% over 29bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC522100.2810048574388783RNA PCR Primer, Index 1 (100% over 24bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG515000.27718349278111926RNA PCR Primer, Index 1 (100% over 29bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT500560.26941159057576125RNA PCR Primer, Index 8 (97% over 36bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAA499890.26905098292495866RNA PCR Primer, Index 13 (100% over 40bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC493220.26546105302816236RNA PCR Primer, Index 1 (100% over 31bp)
TGAGAACTGAATTCCATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC480630.2586848585153191RNA PCR Primer, Index 1 (100% over 31bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG464740.25013253676717934RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT435850.23458335015272003RNA PCR Primer, Index 1 (100% over 30bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT418430.22520755123185188RNA PCR Primer, Index 8 (97% over 36bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG409410.2203528034553748No Hit
TGTAAACATCCTTGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC403970.21742488461900725RNA PCR Primer, Index 1 (100% over 31bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA401260.2159663074045668RNA PCR Primer, Index 1 (100% over 23bp)
TCTCACACAGAAATCGCACCCGTGGAATTCTCGGGTGCCAAGGAACTCCAG396590.21345281825643508RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT394310.2122256758029575RNA PCR Primer, Index 1 (100% over 30bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA369470.19885628170454392RNA PCR Primer, Index 1 (100% over 34bp)
TGAGAACTGAATTCCATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG363570.19568078149598353RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG347160.18684858515319097RNA PCR Primer, Index 1 (100% over 29bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA331210.17826397018259127RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG330510.17788721592055867RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG324650.17473324452697156RNA PCR Primer, Index 1 (100% over 29bp)
TGGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG318890.1716330951708177Illumina Small RNA Adapter 2 (100% over 21bp)
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAAC299790.16135308602107135RNA PCR Primer, Index 1 (100% over 24bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGGA299040.16094942074032212RNA PCR Primer, Index 1 (100% over 22bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC294520.15851666464834027RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT287740.15486753051036747RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA281250.15137448028095102RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACTC279760.15057253192319595RNA PCR Primer, Index 1 (100% over 26bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA277310.14925389200608188No Hit
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT273850.14739164951089223RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG271010.14586310364778857RNA PCR Primer, Index 1 (100% over 29bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAA270000.145319501069713RNA PCR Primer, Index 1 (100% over 23bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA267930.1442053848948452RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT265680.1429943890525976RNA PCR Primer, Index 1 (100% over 30bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC259060.13943137017451795RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA257220.13844104468574658RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTCC252990.13616437250232108RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGGA249070.13405454863493857RNA PCR Primer, Index 1 (100% over 22bp)
TGAGAACTGAATTCCATAGGCAATGGAATTCTCGGGTGCCAAGGAACTCCA247830.13338715537076656RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCACGGAACTCCAGTCACAGTCAAA247340.13312342738734376RNA PCR Primer, Index 13 (97% over 40bp)
TTCAAGTGATGTCATCTTACTACTGAGATGGAATTCTCGGGTGCCAAGGAA240490.1294366178231677RNA PCR Primer, Index 1 (100% over 23bp)
TAATGTGAATTGCAGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA239560.1289360728750387RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG232430.1250985616060496RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT229980.12377992168893553RNA PCR Primer, Index 1 (100% over 30bp)
ATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC225950.12161089358037648RNA PCR Primer, Index 1 (100% over 31bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC225260.12123952152208722RNA PCR Primer, Index 1 (100% over 33bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGCATTCTCGGGTGCCAAGGAAC223060.12005543669855623RNA PCR Primer, Index 1 (95% over 24bp)
TGAGAACTGAATTCCATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCCA221230.11907049341352816RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG218330.11750965432796459RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT218250.11746659669801801RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGATGGAATTCTCGGGTGCCAAGGAACTCC216830.11670232376646617RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC211090.11361293881779896RNA PCR Primer, Index 1 (100% over 33bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT208880.11242347179052463RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGATGGAATTCTCGGGTGCCAAGGAACTCCA205060.11036746996057535RNA PCR Primer, Index 1 (100% over 28bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGCATTCTCGGGTGCCAAGGAAC202340.10890351054239159RNA PCR Primer, Index 1 (95% over 24bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT200630.1079831537022834RNA PCR Primer, Index 1 (100% over 30bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAG199900.10759025282902084RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT197050.10605632476217387RNA PCR Primer, Index 1 (100% over 30bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG196900.10597559170602404No Hit
AACATTCAACGCTGTCGGTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCA195870.1054212247204618RNA PCR Primer, Index 1 (100% over 28bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA186410.10032965997927852RNA PCR Primer, Index 1 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph