FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001283749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001283749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15838816
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC211220413.335618015892098RNA PCR Primer, Index 3 (100% over 51bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA10788626.811506617666371RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC2304591.455026688863612RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2257861.425523220927625RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1804101.1390371603534002RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG1637061.0335747318486432RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1611841.0176518244798096RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1587061.0020067156534933RNA PCR Primer, Index 1 (100% over 29bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1553750.9809761032642843RNA PCR Primer, Index 1 (100% over 32bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC1488000.9394641619676622RNA PCR Primer, Index 3 (100% over 42bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1385750.8749075688485807RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC1369910.864906821317957RNA PCR Primer, Index 1 (100% over 27bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1307910.8257624812359712RNA PCR Primer, Index 1 (100% over 29bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA1243870.785330166093223RNA PCR Primer, Index 1 (100% over 28bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1130440.713714964552906RNA PCR Primer, Index 3 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA1087390.6865349026088818RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1085040.6850512058477098RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC992540.6266503758866824RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC982920.6205766895707356RNA PCR Primer, Index 1 (100% over 27bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG964200.6087576243072714RNA PCR Primer, Index 1 (100% over 29bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT938950.5928157761287207RNA PCR Primer, Index 1 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC894980.5650548626867059RNA PCR Primer, Index 1 (100% over 27bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC892590.5635459115125777RNA PCR Primer, Index 1 (100% over 31bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC867810.5479008026862614RNA PCR Primer, Index 1 (100% over 33bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC800750.5055617793653263RNA PCR Primer, Index 3 (100% over 50bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC794280.5014768780696739RNA PCR Primer, Index 1 (100% over 33bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG760070.47987804138895235RNA PCR Primer, Index 1 (100% over 29bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT716160.45215500956637167RNA PCR Primer, Index 3 (100% over 43bp)
TTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA699860.44186383628675274RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG680610.42971015005162RNA PCR Primer, Index 3 (100% over 37bp)
TACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA639620.4038306903748361RNA PCR Primer, Index 3 (100% over 36bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG612430.38666400316791355RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT611580.38612734689259604RNA PCR Primer, Index 1 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG561140.35428153215492875RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG525700.3319061222758065RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCCA489310.3089309200889763RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC488180.30821748292296597RNA PCR Primer, Index 1 (100% over 27bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT465770.29406869806429975RNA PCR Primer, Index 3 (100% over 34bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG408620.2579864555532434RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA406480.25663534446009095No Hit
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG375170.2368674527186881RNA PCR Primer, Index 3 (100% over 37bp)
TAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT368650.23275098340684053RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA353120.22294595757662694No Hit
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG325020.20520473247495266RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC324700.20500269717130373RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG321570.2030265393574873RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA313520.19794408875006816RNA PCR Primer, Index 3 (100% over 40bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT309060.19512822170546082RNA PCR Primer, Index 1 (100% over 30bp)
CAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG308370.19469258308196774RNA PCR Primer, Index 3 (100% over 38bp)
CAACGGAATCCCAAAAGCAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCA305700.19300685101714674RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG305320.1927669340940636RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA297440.18779181474170797RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA296160.18698367352711212RNA PCR Primer, Index 3 (100% over 36bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC266750.16841536640112492RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA263360.16627505490309377RNA PCR Primer, Index 1 (100% over 28bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT253330.1599425108543467RNA PCR Primer, Index 3 (100% over 41bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT246230.1554598525546354RNA PCR Primer, Index 3 (100% over 34bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA244770.154538066481737RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA243880.15397615579346335RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG233930.14769412057062853RNA PCR Primer, Index 1 (100% over 29bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA232570.14683547053012044RNA PCR Primer, Index 1 (100% over 22bp)
TTCAAGTAATCCAGGATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG229560.1449350759551724RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCCA228910.14452469174463548No Hit
AGTTCTACAGTCCGACGATCTGGACTTCTCGGGTGCCAAGGAACTCCAGTC208860.13186591725038033RNA PCR Primer, Index 1 (96% over 31bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT206060.13009810834345192RNA PCR Primer, Index 1 (97% over 35bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCCG201800.12740851336362516RNA PCR Primer, Index 3 (100% over 51bp)
TGAGAACTGAATTCCATAGGCTGGTGGAATTCTCGGGTGCCAAGGAACTCC200790.12677083943648312RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG196110.1238160731206171RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG192040.12124643660233189RNA PCR Primer, Index 1 (100% over 29bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC189810.11983850308002819RNA PCR Primer, Index 1 (100% over 31bp)
TGAGAACTGAATTCCATGGGTTGTGGAATTCTCGGGTGCCAAGGAACTCCA187550.11841162874800742RNA PCR Primer, Index 1 (100% over 28bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC176370.1113530203267719RNA PCR Primer, Index 3 (100% over 44bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC174450.11014080850487815RNA PCR Primer, Index 1 (100% over 27bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTCC167810.10594857595416222RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC166310.10500153546830772RNA PCR Primer, Index 1 (100% over 24bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC166210.10493839943591743RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA160370.10125125514432393RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph