FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001299822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001299822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365309
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT10339828.304257491602996TruSeq Adapter, Index 20 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG13140.35969549066680534TruSeq Adapter, Index 20 (97% over 43bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCC5600.15329488186713167TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATATCGTAT4230.11579238398177981TruSeq Adapter, Index 20 (97% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)