FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001299824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001299824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442725
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT8615319.45970975210345TruSeq Adapter, Index 21 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG5540.12513411259811397TruSeq Adapter, Index 21 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATATCGTAT5240.11835789711446157TruSeq Adapter, Index 21 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)