FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001299838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001299838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102196
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC50304045.63979546287593TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGGCATCTCGTATGC26840.2435138577893587TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGGCATCTCGTATGC22090.20041807446225537TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGCATCTCGTATGC19560.1774638993427666TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCATCTCGTATGC17800.16149577752051358TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATGCATCTCGTATGC14430.130920453349495TruSeq Adapter, Index 3 (98% over 50bp)
GATCGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC13130.11912581791260356TruSeq Adapter, Index 3 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGGCATCTCGTATGC13040.11830926622851108TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGACATCTCGTATGC13020.11812781029871275TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGGCATCTCGTATGC11560.10488152742343466TruSeq Adapter, Index 3 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)