FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001299850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001299850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306997
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC82392263.03931837640025TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC67550.5168336270090903TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCAGATCTCGTATGC48710.3726863948425283TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAGATCTCGTATGC34400.26319876786251234TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCAGATCTCGTATGC34380.26304574532305736TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCACATCTCGTATGC29200.22341290760422555TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAAGATCTCGTATGC22110.16916641736744614TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATTTCGTATGC21240.16250993690115587TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTAGATCTCGTATGC20370.1558534564348656TruSeq Adapter, Index 9 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC19090.14606001390974885TruSeq Adapter, Index 9 (98% over 50bp)
GATCGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC19010.14544792375192903TruSeq Adapter, Index 9 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCAGATCTCGTATGC17340.1326705417074408TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCAGATCTCGTATGC14860.11369574681502712TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC13550.10367277048072797TruSeq Adapter, Index 9 (97% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)