FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001299884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001299884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900981
Sequences flagged as poor quality0
Sequence length138-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT448454.977352463592462TruSeq Adapter, Index 27 (97% over 39bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT90541.0049046539272193TruSeq Adapter, Index 27 (97% over 36bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT9640.10699448712015015TruSeq Adapter, Index 27 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)