FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001299892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001299892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993150
Sequences flagged as poor quality0
Sequence length137-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT152071.5311886421990635TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG64050.6449176861501283TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT21040.21185118058702107TruSeq Adapter, Index 6 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT19370.19503599657654935TruSeq Adapter, Index 3 (97% over 34bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG18550.1867794391582339TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT11430.11508835523334844TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)