FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001299960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001299960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054792
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT32450.3076435922911816TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGAT16700.1583250536598685TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT15700.14884451152454703TruSeq Adapter, Index 3 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGAT12140.1150937815228026TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGTAT11840.11224961888220616TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGTT11020.10447557433124256TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)