FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001299966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001299966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012271
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT109071.0774782642197593TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT37280.3682808259843461TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTGGTAT11400.11261806373984833TruSeq Adapter, Index 3 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT10270.10145504514107388TruSeq Adapter, Index 10 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)