FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001299976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001299976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533136
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT14360.2693496593739684TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGAT8050.15099336754599202TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT7410.13898892590258394TruSeq Adapter, Index 3 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGAT5600.10503886437982053TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGTAT5440.10203775396896851TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)