FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001300011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324088
Sequences flagged as poor quality0
Sequence length138-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185441.4005111442743987No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA38570.2912948384095317No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA35920.2712810628900798No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA26730.20187479986224482No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG20800.1570892569073959No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA19770.14931031774323159No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT16840.12718187915002627No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA15170.11456942438871133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)