FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856676
Sequences flagged as poor quality0
Sequence length137-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130961.5286992982177627No Hit
AGTGGGATTCCTGCTGTCAGTTAAGGAGATTTTGGGCTGAGACAATGGGG15520.1811653413892767No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA12030.14042648562583754No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA11360.13260555916122316No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA9800.11439564082570307No Hit
AGTGGGATTCCTGCTGTCAGTTAAGGAGATTTTGGGCTGAGACGATGGGG9670.1128781476310764No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA9560.11159411492793075No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTTTCTTTTCATAGAGTAGTTTGGA9030.10540741190368354No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT9000.10505722116646199No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)