FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625083
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG15030.24044806849650366Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG13100.20957216881598123Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG6980.11166517086530908Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG6460.10334627561459839No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG6370.10190646682120615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)