FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001300715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340586
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG13500.3963756584240104Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG9480.2783437956933051Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG6070.17822224049138835Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG4850.14240162543381113No Hit
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG4730.13887828624781995Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGTGGAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG4170.12243603671319432No Hit
GATCGGAAGAGCGTCGGGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG4170.12243603671319432Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG4160.12214242511436173Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGGCGCCG4070.11949992072486833Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG3900.11450852354471411Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGGAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG3780.11098518435872291No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG3770.1106915727598903No Hit
GATCGGAAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG3750.1101043495622251No Hit
GATCGGAAGAGCGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG3630.1065810103762339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)