FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509845
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG13680.2683168413929724Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG9690.19005776265335542Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG6070.1190557914660338No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG5410.1061106806970746No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG5300.1039531622355814Illumina Single End PCR Primer 1 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)