FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616819
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC16891927.38550531030983TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCGTATGC8580.13910077348460406TruSeq Adapter, Index 3 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT7730.12532039382703838Illumina Single End PCR Primer 1 (96% over 30bp)
TAACTGACAGCAGGAATCCCACTAGATCGGAAGAGCACACGTCTGAACTC7170.1162415554644069Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGGGATTCCTGCTGTCAGTTAAGATCGGAAGAGCACACGTCTGAACTC6960.11283699107842009Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT6200.1005157104434202TruSeq Adapter, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)