FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616819
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG14720.23864375124631373No Hit
GATCGGAAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG14460.23442857629223485No Hit
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG13680.2217830514299981Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG11500.18644043066118263No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG10070.1632569684137486Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG8800.1426674599842093Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGGGCCG8680.14072199462078827No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG8460.13715530812118304No Hit
GATCGGGAGAGCGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG8290.1343992321896699No Hit
GATCGGGAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG8030.13018405723559098No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG7690.12467190537256473No Hit
GATCGGAAGAGCGGCGTGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG7440.12061885253210422No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTA7350.11915975350953846Illumina Single End PCR Primer 1 (97% over 34bp)
AGTGGGATTCCTGCTGTCAGTTAAGATCGGAAGAGCGTCGTGTAGGGAAA6950.11267486896480168Illumina Single End PCR Primer 1 (100% over 27bp)
TAACTGACAGCAGGAATCCCACTAGATCGGAAGAGCGTCGTGTAGGGAAA6730.10910818246519643Illumina Single End PCR Primer 1 (100% over 27bp)
GATCGGGAGAGCGGCGGGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG6550.10618998442006489No Hit
GATCGGAAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGGGCCG6480.10505512962473594No Hit
GATCGGGAGAGCGGCGTGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG6310.10229905369322281No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)