FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624032
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG18840.30190759448233423No Hit
GATCGGGAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG10370.16617737551920414No Hit
GATCGGAAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG9930.159126455053587No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGGGCCG9570.15335752012717296No Hit
GATCGGGAGAGCGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG9470.15175503820316907No Hit
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG8700.13941592738833908Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGGGGCGGGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG8350.13380724065432542No Hit
GATCGGGAGAGCGGCGTGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG7980.12787805753551099No Hit
GATCGGGAGAGGGGCGTGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG7890.1264358238039075No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG7550.12098738526229424Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGGAGAGCGGCGGGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG6950.11137249371827086No Hit
GATCGGGAGAGCGGCGGGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG6850.10977001179426696No Hit
GATCGGGAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG6850.10977001179426696Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG6300.10095636121224552Illumina Single End PCR Primer 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)