FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558167
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG328325.882110551143295Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG84801.5192585731510462Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG39420.7062402470945075Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG30810.5519853377215063Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG27660.4955506147801643Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC16140.2891607708803996Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG14660.26264540899049926Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGGCG14420.2583456205759208Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG14210.2545833057131647Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGGGCCG13850.24813362309129705Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGCCG13410.24025067766456992Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG11420.20459826539369041Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGGGCCG9270.16607932751309196Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG8780.15730059283332765Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG8560.15335912011996408Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG8080.14475954329080723Illumina Single End PCR Primer 1 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)