FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001300812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855745
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC39579046.25092755435322TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGCATCTCGTATGC37830.44207094403122427TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGAATCTCGTATGC37030.43272236472313597TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGGATCTCGTATGC27310.31913712612986345TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATTTATCTCGTATGC16990.19854045305552473TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTGTATCTCGTATGC15120.17668814892286838TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGTATCTCGTATGC13470.15740670409993632TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTGTATCTCGTATGC11130.1300621096237781TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTATCTCGTATGC8950.10458723100923756TruSeq Adapter, Index 2 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)