FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842305
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC39887547.355174194620716TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGCATCTCGTATGC33560.39843049726642965TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGAATCTCGTATGC30260.3592522898475018TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGGATCTCGTATGC24250.2879004636087878TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATTTATCTCGTATGC17330.2057449498697028TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTGTATCTCGTATGC15460.18354396566564368TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGTATCTCGTATGC12820.15220139973050142TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTGTATCTCGTATGC10210.12121499931734943TruSeq Adapter, Index 2 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)