FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001300815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842305
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG20727824.608425689031883Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG274573.2597455790954584Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG252953.0030689595811495Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG212142.518565127833742Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG174572.0725271724612817Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG130601.5505072390642345Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC66490.789381518571064Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG47900.5686776167777705Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG43940.5216637678750571Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG35880.4259739643003425Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG35450.4208689251518156Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG33580.3986679409477565Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGACG29800.35379108517698454Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG16310.1936353221220342Illumina Single End PCR Primer 1 (100% over 26bp)
GATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG16130.19149832899009267Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCC13670.16229275618689193Illumina Single End PCR Primer 1 (97% over 49bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG13130.15588177679106738Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATATCGGTGGGCGCCG12820.15220139973050142Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG12170.14448448008737927Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG12110.14377214904339877Illumina Single End PCR Primer 1 (100% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGGCG11680.1386671098948718Illumina Single End PCR Primer 1 (97% over 47bp)
GCTCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG11160.1324935741803741Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC10090.11979033722938841Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG9370.11124236470162234Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCC8660.10281311401451967Illumina Single End PCR Primer 1 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)