FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584349
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG205783.5215256636017176Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG53810.9208538048323861Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG27580.4719782184961384Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG20510.3509888782217476Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG17860.305639266944925Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC10670.18259635936743282Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGGCG10080.1724996534605176Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGGGCCG8460.14477649486864871Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGCCG8000.13690448687342666Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG7810.1336530053101828Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG7280.12458308305481826Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG6030.10319175698084534Illumina Single End PCR Primer 1 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)