FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574049
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG191503.3359521573942295Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG22810.39735283921755804Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG21680.3776681084715765Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG18510.3224463416886015Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG15910.27715404085713935Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG10130.1764657720856582Illumina Single End PCR Primer 1 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)