FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001300825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144796
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG28693925.06464033766715Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG708156.185818259323058Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG335062.926809667399257Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG256572.2411853290892Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG256002.2362062760526764Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC147741.2905356063438378Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG127901.1172296199497551Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG124921.0911987812675796Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGGGCCG116601.0185220772958676Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGGCG112960.9867260193082437Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGCCG111060.9701291758531652Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG107110.9356252118281335Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGGGCCG80760.7054531986484929Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG80340.7017844227268439Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG78280.6837899503492326Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG65300.5704073040087492Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG40510.3538621728238044Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGGGGCG39930.3487957679796226Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGACG35980.31429180395459105Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG35440.3095748063410424Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG33510.29271590746298903Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGGGGCG30670.2679079940880296Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG30490.2663356615501801Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCC28000.24458506144326148Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC21640.1890293117725778Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG19930.1740921526630072Illumina Single End PCR Primer 1 (100% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCC19620.17138424662559967Illumina Single End PCR Primer 1 (97% over 49bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATATCGGTGGGCGCCG19520.1705107285490166Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTGGCCG18520.16177554778318584Illumina Single End PCR Primer 1 (97% over 45bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGGGGCG17870.1560976802853958Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGGCG17200.15024510917228923Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGGCG16450.14369372359791616Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGGCG16320.14255815009835815Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGACG16300.14238344648304152Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG16190.14142257659880014Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGTGCCG15860.13853996694607598Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG15760.1376664488694929Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCC15440.13487119102442707Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGGGGCG15300.13364826571721075Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGGGGCG14930.13041624883385336Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGTGGGGGCCG14370.12552454760498816Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG13690.11958462468422322Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGGCG13400.11705142226213229Illumina Single End PCR Primer 1 (97% over 47bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGTGCCG12640.1104126848801009Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG12540.10953916680351784Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC12270.10718066799674353Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGCCC11890.10386129930572782Illumina Single End PCR Primer 1 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)