FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001300826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124910
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC73013864.90634806340063TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGC52470.4664373149851988TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC40930.3638513303286485TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAAGTGATCTCGTATGC20640.1834813451742806TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGATCTCGTATGC18170.16152403303375382TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGATCTCGTATGC16800.1493452809558098TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGTGATCTCGTATGC15190.13503302486421137TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTGATCTCGTATGC11870.10551955267532513TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGTGATCTCGTATGC11530.10249708865598137TruSeq Adapter, Index 5 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)