FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747363
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG13911818.614515302470153Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG348874.668012732768414Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG166212.2239527512065758Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG128671.7216533331192472Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG120881.6174201826956915Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC71020.9502744984699538Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG65110.8711964600869991Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG61700.8255693685665467Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGGGCCG60120.8044283701494453Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGGCG58610.7842239982444942Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGCCG52800.7064839977360399Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG51620.690695150816939Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGGGCCG38720.5180882650064292Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG37930.5075177657978787Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG35740.47821473634632705Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG35390.4735316037855768Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG19590.2621216196145648Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGACG17640.23602988106181333Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG17120.22907208411441296Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG16630.22251569852936257Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGGGGCG16460.22024103414271246Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG14620.19562113725191105Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCC12750.17059982899875964Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC10430.13955735031035787Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCC9370.1253741488406571Illumina Single End PCR Primer 1 (97% over 49bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG9370.1253741488406571Illumina Single End PCR Primer 1 (100% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATATCGGTGGGCGCCG9190.12296568066655694Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTGGCCG8780.11747972538110664Illumina Single End PCR Primer 1 (97% over 45bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGGCG8750.11707831401875661Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGGCG8700.11640929508150658Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8180.10945149813410618Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG7920.10597259966040598Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCC7870.10530358072315595Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGGCG7800.1043669542110059Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGACG7600.10169087846200575Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGTGGGGGCCG7590.10155707467455574Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGTGCCG7520.1006204481624057Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)