FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001300843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902665
Sequences flagged as poor quality0
Sequence length32-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG13090.1450150388017703Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG13020.14423955731085175Illumina Single End PCR Primer 1 (95% over 23bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11340.12562800152880635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)