FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001300845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335241
Sequences flagged as poor quality0
Sequence length32-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37010.27717842696561895No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG23560.1764475476711695Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG20780.15562733618874797Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG16670.12484637604747008Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG14960.11203969920036906No Hit
GATCGGAAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG14900.11159034211801465No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG13860.10380148602387135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)