FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001300856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261923
Sequences flagged as poor quality0
Sequence length32-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC4660.17791488338175723TruSeq Adapter, Index 3 (100% over 50bp)
TAACCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCT3740.14279005661969357No Hit
TAAACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCA2800.10690164666715028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)