FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001300868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642131
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC17320.26972689373352166TruSeq Adapter, Index 7 (100% over 50bp)
TAACCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCT14020.21833551097828946No Hit
TAAACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCA10590.16491961920542694No Hit
TAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAA7150.11134799596966974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)