FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001300880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045829
Sequences flagged as poor quality0
Sequence length32-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC19744518.879281412162026TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC52470.5017072580699139TruSeq Adapter, Index 9 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20040.19161832383687963No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAGATCTCGTATGC12780.1221997095127406TruSeq Adapter, Index 9 (98% over 50bp)
TAACCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCT11870.11349847824070666No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCAGATCTCGTATGC11590.11082117631085005TruSeq Adapter, Index 9 (98% over 50bp)
TAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAA10830.1035542139298107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)