FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001300886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856261
Sequences flagged as poor quality0
Sequence length32-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC29352715.81280865136961TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGAAATCTCGTATGC37380.20137254405495777TruSeq Adapter, Index 12 (98% over 50bp)
TAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAA34040.1833793846878214No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC31490.16964209235662442TruSeq Adapter, Index 12 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22980.12379724618466909No Hit
TAACCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCT22140.1192720204755689No Hit
TAAACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCA21400.11528551211279017No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCAATCTCGTATGC19140.10311050008592541TruSeq Adapter, Index 12 (98% over 50bp)
TAATCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCT18660.10052465682358246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)