FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001300914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105865
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC11598810.4884411750078TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC23660.21395016570738742TruSeq Adapter, Index 8 (98% over 50bp)
TAACCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCT20320.18374756412401153No Hit
TAAACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCA16320.14757678378463918No Hit
TAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAA14980.13545957237094944No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)