FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001300932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369035
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT12590.34116005256953946TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGTT7130.1932066064194453TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGAT6010.16285718156814394TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT4870.13196580270164077TruSeq Adapter, Index 6 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGTT3990.10811982603276113TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGGT3960.10730689500995841TruSeq Adapter, Index 6 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGAT3730.10107442383513758TruSeq Adapter, Index 3 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)