FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001300944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251977
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT45501.805720363366498TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT4380.17382538882517054TruSeq Adapter, Index 6 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA3330.1321549189013283No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA2860.11350242284017986No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT2750.10913694503863448TruSeq Adapter, Index 3 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)