FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001300962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457638
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT43980.9610215934865548TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT18850.4118976133974888TruSeq Adapter, Index 8 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA6290.13744488001433447No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGTAT5590.12214894742132427TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGGAT5310.1160305743841202TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTGGTAT5090.11122328128345986TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)