FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001300964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208129
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT3760.18065718857055TruSeq Adapter, Index 8 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA3170.15230938504485197No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGAT2790.1340514776893177TruSeq Adapter, Index 8 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA2770.13309053519692116No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGTT2400.11531309908758511TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT2310.11098885787180066TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)