FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001300968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252612
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT33471.3249568508226055TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT5700.2256424872927652TruSeq Adapter, Index 8 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA4750.18803540607730432No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA4410.1745760296422973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)