FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001300993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185477
Sequences flagged as poor quality0
Sequence length139-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305552.5774435100807525No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA21780.18372351382608013No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA17260.14559540168219207No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA16520.13935318863208648No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT16280.13732868710232252No Hit
AGTGGGATTCCTGCTGTCAGTTAATTTCCATTCCATTCGAGACCTTTCCA16260.13715997864150886No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG15820.13344839250360824No Hit
AGTGGGATTCCTGCTGTCAGTTAAATGGAAATGAATCAAATTGAATAGCA15660.13209872481709894No Hit
AGTGGGATTCCTGCTGTCAGTTAAGAAGCGCAGCCTAATAACGTGTGGGC14660.12366330177641574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT13760.11607142103980087No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCGATTGCAA13600.11472175335329154No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA12790.10788906069033816No Hit
AGTGGGATTCCTGCTGTCAGTTAACTAGTAATTTGAAGAGTTGGCATTTG12670.10687680992545617No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT12000.10122507648819842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)