FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001300995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185674
Sequences flagged as poor quality0
Sequence length138-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275142.320536673655659No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA20520.17306612104170288No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG17710.1493665206456412No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT16830.1419445817315721No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA16780.14152288065690907No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT16390.1382336122745375No Hit
AGTGGGATTCCTGCTGTCAGTTAAATGGAAATGAATCAAATTGAATAGCA16230.13688416883561585No Hit
AGTGGGATTCCTGCTGTCAGTTAATTTCCATTCCATTCGAGACCTTTCCA16070.1355347253966942No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA15810.1333418798084465No Hit
AGTGGGATTCCTGCTGTCAGTTAAGAAGCGCAGCCTAATAACGTGTGGGC14540.12263067251200584No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCGATTGCAA13330.11242550650516078No Hit
AGTGGGATTCCTGCTGTCAGTTAACTAGTAATTTGAAGAGTTGGCATTTG12560.10593130995535029No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA12190.10281072200284395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)