FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001300997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001300997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265998
Sequences flagged as poor quality0
Sequence length138-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233231.8422619940947773No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA21740.1717222302088945No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA20240.15987387025887875No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG17750.14020559274185268No Hit
AGTGGGATTCCTGCTGTCAGTTAAGAAGCGCAGCCTAATAACGTGTGGGC15440.12195911841882848No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA14900.11769370883682281No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT13680.10805704274414335No Hit
AGTGGGATTCCTGCTGTCAGTTAAATGGAAATGAATCAAATTGAATAGCA12970.10244881903446926No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)