FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001301001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272285
Sequences flagged as poor quality0
Sequence length138-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG194191.5263089637934897No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA21590.16969468318812217No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA20720.1628565926659514No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG17240.13550423057726846No Hit
AGTGGGATTCCTGCTGTCAGTTAAGAAGCGCAGCCTAATAACGTGTGGGC15530.1220638457578294No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA14670.11530435397729283No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT13760.10815186848858549No Hit
AGTGGGATTCCTGCTGTCAGTTAACTAGTAATTTGAAGAGTTGGCATTTG12910.10147097544968306No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)