FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001301009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434907
Sequences flagged as poor quality0
Sequence length139-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183474.218603057665201No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA7680.1765894777504156No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT5380.12370460811161926No Hit
AGTGGGATTCCTGCTGTCAGTTAAATGGAAATGAATCAAATTGAATAGCA4970.11427730526296427No Hit
AGTGGGATTCCTGCTGTCAGTTAATTTCCATTCCATTCGAGACCTTTCCA4890.11243783153639744No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCGATTGCAA4890.11243783153639744No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA4840.11128816045729317No Hit
AGTGGGATTCCTGCTGTCAGTTAAGGAGATTTTGGGCTGAGACAATGGGG4650.10691941035669696No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT4370.10048125231371305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)