FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001301050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785710
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT143431.8254826844510061TruSeq Adapter, Index 7 (97% over 35bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT64870.8256226852146467TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT16410.20885568466737092TruSeq Adapter, Index 7 (97% over 35bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT13740.1748736811291698TruSeq Adapter, Index 9 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT8890.11314607170584566TruSeq Adapter, Index 9 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)