FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001301084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422650
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT6664615.768602862888914TruSeq Adapter, Index 7 (97% over 38bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT6500.15379155329468827TruSeq Adapter, Index 9 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG4850.11475215899680587TruSeq Adapter, Index 7 (97% over 37bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4550.1076540873062818TruSeq Adapter, Index 9 (97% over 35bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4350.10292203951259907TruSeq Adapter, Index 9 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)