FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001301088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433431
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT7020916.19842604705247TruSeq Adapter, Index 7 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG5080.11720435317270798TruSeq Adapter, Index 7 (97% over 37bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4860.11212857409829938TruSeq Adapter, Index 9 (97% over 35bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4570.10543777440930621TruSeq Adapter, Index 9 (97% over 35bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4420.10197701594948216TruSeq Adapter, Index 9 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)